SALMON
Salmon is a fast program to produce highly-accurate, transcript-level quantification estimates from RNA-seq data. Salmon achieves its accuracy and speed via a number of different innovations, including the use of quasi-mapping (accurate but fast-to-compute proxies for traditional read alignments), and massively-parallel stochastic collapsed variational inference. The result is a versatile tool that fits nicely into many pipelines. For example, you can choose to make use of the quasi-mapping algorithm by providing Salmon with raw sequencing reads, or, if it is more convenient, you can provide Salmon with regular alignments (e.g. an unsorted BAM file produced with your favorite aligner), and it will use the same fast, state-of-the-art inference algorithm to estimate transcript-level abundances for your experiment.
Note
Salmon works by (quasi)-mapping sequencing reads directly to the transcriptome. This means the Salmon index should be built on a set of target transcripts, not on the genome of the underlying organism. If indexing appears to be taking a very long time, or using a tremendous amount of memory (which it should not), please ensure that you are not attempting to build an index on the genome of your organism!
License
Free to use and open source under GNU GPLv3.
Available
- Puhti: 0.14.2, 0.99.0b2, 1.1.0, 1.4.0, 1.9.0
Usage
On Puhti, the salmon command is activated by loading the Salmon module.
module load salmon
For usage help, use the command:
salmon --help
Note
Loading the biokit module will load an older version of Salmon that comes with Trinity. To use the latest version of Salmon, load the Salmon module directly as above.